Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry.

Combined fluorescence in situ hybridization and immunocytochemistry double labeling in whole-mount Manduca sexta tissue.

Methods for double detection of gene expression. Combined in situ hybridization and immunocytochemistry of histochemistry.

The distribution of two different molecules can be analyzed within the same embryo using the procedures described below. The protocol for combined whole mount in situ hybridization and immunocytochemistry allows for simultaneous detection of mRNA and protein. The protocol for combined whole mount in situ hybridization and β-galactosidase staining allows for simultaneous detection of mRNA and tr...

Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level.

Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the...

Double labeling for whole-mount in situ hybridization in mouse.

1.Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S517S. 2.Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK. 3.Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cel...

Autoradiographic visualization of 35S-labeled cRNA probes combined with immunoperoxidase detection of choleragenoid: a double-labeling light microscopic method for in situ hybridization and retrograde tract tracing.

We describe a protocol for simultaneous light microscopic visualization of a neuron's efferent projections and its expression of mRNA. We have combined immunohistochemical visualization of the retrograde marker cholera toxin subunit B (CTb) with autoradiographic visualization of 35S-labeled cRNA probes. Injections of CTb were made into rat brain. Immunoreactivity for CTb was demonstrated by mod...